Organoid compositions for the production of hematopoietic stem cells and derivatives thereof
AssigneeChildren's Hospital Medical Center
InventorsTakanori Takebe, James M. Wells, Kyle Lewis, Jorge Orlando Munera
Life of this patent
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Abstract
The instant disclosure relates to compositions derived from precursor cells, and methods of using such compositions, for the manufacture of hematopoietic stem cells (HSCs) or derivative immune cells. More particularly, methods for obtaining hematopoietic stem cells from organoid tissue or cultures comprising organoids are disclosed, wherein the organoid tissue or cultures comprise liver or colonic tissue derived from precursor cells (such as embryonic stem cells or induced pluripotent stem cells), via directed differentiation.
Claims26
A method of making hematopoietic stem cells (HSCs), or a derivative cell thereof, comprising
- a. activating a wnt signaling pathway and a FGF signaling pathway in a definitive endoderm derived from a pluripotent precursor cell for a first period of time until foregut cells are formed;
- b. culturing said foregut cells in the absence of retinoic acid for a second period of time to form a liver organoid producing hematopoietic stem cells, or a derivative cell thereof, and
- c. isolating or harvesting the hematopoietic stem cells, or the derivative cell thereof, from the liver organoid, wherein said derivative cell is selected from myeloid cells, lymphoid cells, and combinations thereof.
Dependencies are inferred from the claim text.