Lentinula edodes-derived saltiness enhancing peptide, method for preparing the same and use thereof

A method for preparing a Lentinula edodes-derived saltiness enhancing peptide having an amino acid sequence set forth in SEQ ID NO:

• 1, comprising the following steps:
• subjecting a Lentinula edodes fruiting body to a first enzymolysis with a flavourzyme to obtain an enzymolysis extract by flavourzyme;
• subjecting the enzymolysis extract by flavourzyme to a second enzymolysis with a trypsin to obtain an enzymolysis extract by trypsin, and subjecting the enzymolysis extract by trypsin to solid-liquid separation to obtain an enzymolysis extract from Lentinula edodes;
• subjecting the enzymolysis extract from Lentinula edodes to ultrafiltration to obtain an ultrafiltration permeate, and subjecting the ultrafiltration permeate to nanofiltration to obtain a nanofiltration retentate, wherein the ultrafiltration is conducted at a molecular weight cut-off of 3 kDa, and the nanofiltration is conducted at a molecular weight cut-off of 200 Da;
• and
• subjecting the nanofiltration retentate to gel chromatography separation to obtain an eluate, and collecting the eluate of 100 min to 150 min, wherein the eluate comprises the Lentinula edodes-derived saltiness enhancing peptide, and an eluent for the gel chromatography separation is water.