Method for controlling microscopic imaging and corresponding microscope control arrangement and microscope

A microscope control arrangement comprising one or more processors and adapted for controlling microscopic imaging of a microscope,

• wherein the microscope control arrangement is configured for receiving sample information concerning a sample to be imaged and for receiving a focusing request, wherein the sample information comprises a respective sample specification selected from a plurality of predefined sample specifications, wherein the plurality of predefined sample specifications comprises:
• the sample being adherent to a sample carrier for carrying the sample;
• the sample being detached from the sample carrier for carrying the sample;
• the sample having a varying extension in a focal direction;
• the sample being able to move;
• and/or
• the sample having a surface topology varying over time;
• and
• wherein the microscope control arrangement is further configured to activate, upon receipt of the focusing request and the sample information, a predefined focusing setting depending on the sample information received for controlling focusing of the microscope for microscopic imaging of the sample, wherein the activating the predefined focusing setting automatically initiates a focus strategy associated with the respective sample specification, wherein the initiating the focus strategy comprises:
• when the respective sample specification specifies the sample as being adherent to a sample carrier for carrying the sample, using a triangulating autofocusing method for determining and/or maintaining a focus point, the focus point being used for sample imaging,
• when the respective sample specification specifies the sample as being detached from the sample carrier for carrying the sample or having a varying extension in a focal direction, defining a focus map of one or more focus points, or
• when the respective sample specification specifies the sample as being able to move, using an image based autofocusing method by evaluating a stack of images of the sample, each image being captured at a different focal position.

A microscope control arrangement comprising:

• one or more memories for storing computer-executable instructions;
• and
• one or more processors configured to execute the instructions so as to facilitate a method for controlling microscopic imaging of a microscope, the method comprising:
• receiving sample information concerning a sample to be imaged and receiving a focusing request, wherein the sample information comprises a respective sample specification selected from a plurality of predefined sample specifications, wherein the plurality of predefined sample specifications comprises:
• the sample being adherent to a sample carrier for carrying the sample;
• the sample being detached from the sample carrier for carrying the sample;
• the sample having a varying extension in a focal direction;
• the sample being able to move;
• and/or
• the sample having a surface topology varying over time;
• and
• activating, upon receipt of the focusing request and the sample information, a predefined focusing setting depending on the sample information received for controlling focusing of the microscope for microscopic imaging of the sample, wherein the activating the predefined focusing setting automatically initiates a focus strategy associated with the respective sample specification, wherein the initiating the focus strategy comprises:
• when the respective sample specification specifies the sample as being adherent to a sample carrier for carrying the sample, using a triangulating autofocusing method for determining and/or maintaining a focus point, the focus point being used for sample imaging,
• when the respective sample specification specifies the sample as being detached from the sample carrier for carrying the sample or having a varying extension in a focal direction, defining a focus map of one or more focus points, or
• when the respective sample specification specifies the sample as being able to move, using an image based autofocusing method by evaluating a stack of images of the sample, each image being captured at a different focal position.