Capture flow assay device and methods
AssigneeB. G. Negev Technologies and Applications Ltd., At Ben-Gurion University
InventorsRobert Marks, Tim Axelrod, Ariel Kushmaro
Life of this patent
Estimating the term…
Abstract
Disclosed is a device including at least a section 1, a section 2, a section 3 and a section 4 wherein section 2 is coupled to section 1, section 3 is coupled to section 2 and comprises a surface functionalized with an analyte or equivalent thereof, section 4 is coupled to section 3, and section 1, section 2, section 3 and section 4 are arranged along a horizontal axis and in fluid communication allowing lateral flow from section 1 throughout all sections to section 4. Disclosed are also a kit and method for determining and quantifying the presence of an analyte in a sample.
Claims20
A device for detecting an analyte in a sample, the device comprising a section 1, a section 2, a section 3, a section 4, and a calibration area wherein:
- a. said section 2 is coupled to said section 1, said section 2 comprises a surface comprising a recognition molecule having specific affinity to said analyte, wherein said recognition molecule is linked to a reporter molecule, and wherein said reporter molecule generates a trigger whether bound to said analyte or not;
- b. said section 3 is coupled to said section 2 and to said section 4, said section 3 comprises a surface functionalized with said same analyte as present in the sample, wherein binding of said reporter molecule to said analyte in section 3 produces a reduced signal indicating an analyte negative sample;
- and
- c. sections 1 to 4 are arranged along a horizontal axis and in fluid communication allowing lateral flow of fluid from said section 1 through said section 2 and said section 3 to section 4, and said section 4 comprises a surface in contact with a substrate molecule generating a signal in response to said reporter molecule.
A kit for diagnosing an infectious disease in a subject in need thereof, comprising:
- (i) a section 1 comprising a sample collecting surface;
- (ii) a section 2, comprising a surface deposited with a recognition molecule having specific affinity to an analyte linked to a reporter molecule, wherein said reporter molecule generates a chemically and/or an electrically and/or a physically detectable reaction whether bound to said analyte or not;
- (iii) a section 3 functionalized with said analyte, wherein binding of said reporter molecule to said analyte in section 3 produces a reduced signal indicating an analyte negative sample;
- (iv) a section 4 comprising a surface deposited with a substrate molecule generating a signal in response to said reporter molecule;
- and
- (v) a section 5 comprising a surface available for holding excess sample, and wherein the kit-further comprises a calibration area.
Dependencies are inferred from the claim text.