Ribosome variants for sequence defined polymer synthesis
AssigneeNorthwestern University
InventorsMichael Christopher Jewett, Anne E. d'Aquino
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Abstract
Disclosed are engineered or modified E. coli ribosomes and methods, components, compositions, and kits for preparing and identifying engineered or modified E. coli ribosomes. The engineered or modified E. coli ribosomes may be prepared and identified under a set of defined conditions, such as in the presences of a engineered or modified tRNA comprising a non-natural, non-α-amino acid monomer (NNA), in order to obtain an engineered or modified ribosome that utilizes the engineered or modified tRNA as a substrate for synthesizing a polymer comprising the NNA.
Claims8
An engineered Escherichia coli ribosome comprising one or more engineered mutations in ribosomal RNA of the ribosome, wherein at least one of the one or more engineered mutations is selected from the group consisting of A2448C, A2448U, A2448G, A2453U, A2453C, C2456U, C2456G, C2456A, C2496U, C2496G, C2496A, A2497U, A2497C, C2498U, C2499G, U2500A, U2500G, A2567C, G2588U, G2588C, C2606U, C2606A, C2606G, G2607U, and G2607A, wherein the positions are defined relative to a wild-type Escherichia coli 23 S rRNA encoded by SEO ID NO:
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Dependencies are inferred from the claim text.