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US 12,421,522 B2Granted
Modulation of transgene expression in plants
AssigneeCorteva Agriscience LLC
InventorsAndrew J. Bowling, Marcelo Ariel German, Todd P. Glancy, Sandeep Kumar, Heather E. Pence, Andrew E. Robinson, Shreedharan Sriram, Pohao Wang, Carla N. Yerkes
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Abstract
This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.
Claims11
A method to reduce expression of a first aad-12 transgene conferring resistance to a 2,4-D herbicide within a soybean plant, the method comprising:
- (a) introducing into the soybean plant a small RNA target site polynucleotide operably linked to an expression cassette comprising a second transgene, thereby selectively reducing the expression of the first transgene within seed-coat specific plant tissue of the soybean plant and conferring expression of the first transgene in soybean plant tissues comprising above ground tissues of the soybean plant;
- (b) obtaining the soybean plant comprising reduced expression of the first transgene within seed-coat specific plant tissues;
- (c) producing a seed from the soybean plant;
- (d) growing a progeny soybean plant from the seed;
- and
- (e) applying a 2,4-D herbicide to the progeny soybean plant, wherein the above ground tissues of the progeny soybean plant are tolerant to the application of the 2,4-D herbicide.
3 more elements
Dependencies are inferred from the claim text.