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US 12,421,511 B2Granted
Mutant protein for improving malic acid yield
AssigneeTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
InventorsChaoguang Tian, Junqi Zhao, Jingen Li, Lu Zhang
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Abstract
The present invention provides a class of new mutant proteins for increasing malic acid yield. Specifically, the present invention provides a class of new pyruvate carboxylase mutant protein and malic acid transporter mutant proteins or combinations thereof, a preparation method therefor and use thereof in improving malic acid yield.
Claims17
A pyruvate carboxylase mutant protein, wherein the pyruvate carboxylase mutant protein has one or more amino acid mutations at positions corresponding to the amino acid sequence as shown in SEQ ID NO:
- 1 selected from the group consisting of:
- A762D, A762P, A762V, A762T, P826E, P826Q, P826Y, P826T, P826D and P826I;
- wherein the pyruvate carboxylase mutant protein has an activity of carboxylation of pyruvate into oxaloacetate, and wherein the amino acid sequence of the pyruvate carboxylase mutant protein has at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 1.
1 more element
Dependencies are inferred from the claim text.