Serine proteases
AssigneeDanisco US Inc.
InventorsMarc Kolkman, Rie Mejldal, Anja Hemmingsen Kellett-Smith, Lilia Maria Babe
Life of this patent
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Abstract
The present disclosure relates to serine proteases and variants thereof. Compositions containing the serine proteases are suitable for use in cleaning fabrics and hard surfaces, as well as in a variety of industrial applications.
Claims11
A method of cleaning comprising contacting a surface or an item in need of cleaning with a composition comprising a surfactant and a subtilisin comprising a DLGDXXRFGXaGLLXXXXAVX (SEQ ID NO:26) motif, wherein X is any amino acid and Xa is N or S, wherein said subtilisin comprises an amino acid sequence having at least 85% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
- 3, wherein the subtilisin has improved protease activity when compared to protease activity of Bacillus lentus GG36 subtilisin;
- and optionally further comprising the step of rinsing said surface or item after contacting said surface or item with said composition.
A method for producing a subtilisin comprising:
- (a) stably transforming a host cell with an expression vector encoding a subtilisin comprising a DLGDXXRFGXaGLLXXXXAVX (SEQ ID NO:26) motif, wherein X is any amino acid and Xa is N or S, wherein said subtilisin comprises an amino acid sequence having at least 85% amino acid sequence identity to the amino acid sequence of SEQ ID NO:
- 3, wherein the subtilisin has improved protease activity when compared to protease activity of Bacillus lentus GG36 subtilisin;
- (b) cultivating said transformed host cell under conditions suitable for said host cell to produce said subtilisin polypeptide;
- and
- (c) recovering said subtilisin or polypeptide.
Dependencies are inferred from the claim text.