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US 12,421,507 B2Granted
Methods and compositions for optochemical control of CRISPR-CAS9
AssigneesThe Broad Institute, Inc., The Brigham and Womens's Hospital, Inc.
InventorsAmit Choudhary, Debasish Manna, Basudeb Maji, Soumyashree Ashok Gangopadhyay
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Abstract
The disclosure includes non-naturally occurring or engineered DNA- or RNA-guided nuclease systems, comprising CRISPR enzymes associated with at least one destabilization domain (DD) and photocaged stabilization ligands with at least one photocage molecule, along with compositions, systems and complexes involving such systems, nucleic acid molecules and vectors encoding the same, delivery systems involving the same, uses thereof.
Claims19
A CRISPR system comprising:
- a. a CRISPR enzyme comprising one or more destabilizing domains;
- and
- b. one or more photocaged ligands comprising at least one photocage molecule having a chemical structure comprising:
- wherein, upon exposure to UV or visible light, the one or more photocaged ligands can bind to the one or more destabilizing domains.
1 more element
Dependencies are inferred from the claim text.