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US 12,421,503 B2Granted
Ketoreductase polypeptides for the production of azetidinone
AssigneeCodexis, Inc.
InventorsOnorato Campopiano, Emily Mundorff, Birthe Borup, Rama Voladri
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Abstract
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Claims14
An engineered ketoreductase polypeptide having ketoreductase activity,
- wherein said ketoreductase polypeptide is at least 85% identical to SEQ ID NO:4, and
- wherein the ketoreductase polypeptide comprises the following fetures:
- the resiude at the position corresponding to position 153 is alanine or serine,
- the residue at position corresponding to position 202 is valine or leucine, and
- wherein the residue at the position corresponding to position 94 is threonine.
2 more elements
Dependencies are inferred from the claim text.