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US 12,421,500 B2Granted
Hepato-biliary-pancreatic tissues and methods of making same
AssigneeChildren's Hospital Medical Center
InventorsTakanori Takebe, Hiroyuki Koike
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Abstract
Disclosed herein are hepato-biliary-pancreatic organoid (“HBPO” or “HBP organoid”) compositions, and methods of making and using hepato-biliary-pancreatic organoid compositions. The disclosed compositions may have two or more functions selected from hepatic tissue function, biliary tissue function, exocrine pancreatic function, and endocrine pancreatic tissue function. Methods of treating individuals using the hepato-biliary-pancreatic organoid compositions is also disclosed.
Claims17
A method of making a hepato-biliary-pancreatic organoid (HBPO), comprising:
- a) contacting an anterior gut spheroid with a posterior gut spheroid for a first period of time sufficient to form a fused spheroid, wherein the contacting is in a gut growth medium;
- b) culturing the fused spheroid for a second period of time sufficient to form a boundary organoid having a foregut-midgut boundary, wherein culturing the fused spheroid comprises embedding said fused spheroid into a basement membrane matrix;
- and
- c) culturing the boundary organoid of step b) in a second gut growth medium in an air-liquid interface to form the HBPO, wherein said HBPO comprises biliary tissue and pancreatic tissue.
1 more element
Dependencies are inferred from the claim text.