Cardiac fibroblast derived extracellular matrix
AssigneeWisconsin Alumni Research Foundation
InventorsEric Schmuck, Amish Raval, Sushmita Roy, Tianhua Zhou
Life of this patent
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Abstract
The disclosure generally relates to methods, cells, and compositions for preparing cardiac extracellular matrix. In particular, provided herein are methods for preparing a cardiac extracellular matrix using SUSD2 High fibroblasts and SUSD2 High myofibroblasts.
Claims22
A method for preparing a cardiac extracellular matrix, comprising:
- (a) selecting SUSD2 High fibroblasts from a population of cardiac fibroblasts;
- and
- (b) plating the SUSD2 High fibroblasts into a culture having a cell density of 100,000 to 500,000 cells per cm2, wherein the SUSD2 High fibroblasts secrete a cardiac extracellular matrix that is attached to the surface on which the SUSD2 High fibroblasts are plated.
A method for preparing a cardiac extracellular matrix, comprising:
- (a) selecting SUSD2 High myofibroblasts from a population of cardiac myofibroblasts;
- and
- (b) plating the SUSD2 High myofibroblasts into a culture having a cell density of 100,000 to 500,000 cells per cm2, wherein the SUSD2 High myofibroblasts secrete a cardiac extracellular matrix that is attached to the surface on which the SUSD2 High myofibroblasts are plated.
A method of generating a population of SUSD2 High fibroblasts, the method comprising selecting SUSD2 High fibroblasts from a population of cardiac fibroblasts to yield a population of SUSD2 High fibroblasts.
A method of generating a population of SUSD2 High myofibroblasts, the method comprising selecting SUSD2 High myofibroblasts from a population of cardiac myofibroblasts to yield a population of SUSD2 High myofibroblasts.
Dependencies are inferred from the claim text.