Diet controlled expression of a nucleic acid encoding a pro-apoptotic protein
AssigneesInstitut National De Recherche Pour L'agriculture, L'alimentation L'environnement, Centre National De La Recherche Scientifique, Sorbonne Universite, Assistance Publique-Hopitaux de Paris, Institut National De La Sante Et De La Recherche Medicale (Inserm), Institut Du Cerveau Et De La Moelle Epiniere
InventorsPierre Fafournoux, Jacques Mallet
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Abstract
A nucleic acid for the controlled expression of a nucleic acid encoding a pro-apoptotic protein in an individual, including: a regulatory polynucleotide including a minimal promoter and at least one AARE (amino acid response element) nucleic acid, the regulatory polynucleotide being activated in an individual upon consumption of a diet deficient in at least one essential amino acid; and a nucleic acid encoding a pro-apoptotic protein, which is placed under the control of the regulatory polynucleotide.
Claims10
A method for eliminating infused cells which contain a nucleic acid comprising:
- a regulatory polynucleotide comprising a minimal promoter and at least one AARE (amino acid response element) nucleic acid, the regulatory polynucleotide being activated in a human individual upon consumption of a diet deficient in at least one essential amino acid;
- and
- a suicide safety gene selected from the group consisting of:
- BCL2 modifying factor, Apoptosis inducer NBK, Apoptotic protease-activating factor 1 (APAF1, CED4), BAX, Endophilin B1, FADD, CRADD, FAS, Modulator of apoptosis 1, SMAC, Endonuclease G, HRK, Serine protease HTRA2, p53, NOXA, RIPK1, Bcl2 binding component 3, PMAIP1, Tumor necrosis factor receptor superfamily member 10A Precursor (Death receptor 4), caspase 1, caspase 3, caspase 7, caspase 8, and caspase 9, the product of which is associated with the killing of cells, said suicide safety gene being placed under the control of the regulatory polynucleotide, the method comprising:
- a) administering the infused cells to a human individual in need of cancer treatment or regenerative medicine, and then
- b) eliminating the infused cells by inducing programmed cellular death into at least one of the infused cells by submitting the human individual to a diet deficient in at least one essential amino acid.
Dependencies are inferred from the claim text.