# US 12,419,939 B2 · Vaccine and methods for detecting and preventing filariasis

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- Number: US 12,419,939 B2
- Document ID: US12419939B2
- Status: Granted
- Kind: B2
- Application number: 17/798,189
- Filed: 2021-02-12
- Published: 2023-03-23
- Granted: 2025-09-23
- Earliest claimed: 2020-02-13
- Assignees: The Board of Trustees of the University of Illinois
- Applicants: The Board of Trustees of the University of Illinois
- Inventors: Ramaswamy Kalyanasundaram
- Web page: https://patentgrep.com/patent/US12419939B2

## CPC

- A61K 39/0003: {Invertebrate antigens}
- A61P 33/10: Anthelmintics
- A61K 2039/53: {DNA (RNA) vaccination}
- A61K 2039/552: {Veterinary vaccine}
- A61K 2039/55505: {Inorganic adjuvants}
- A61K 2039/55566: {Emulsions, e.g. Freund's adjuvant, MF59}
- A61K 2039/575: {humoral response}
- A61K 2039/70: {Multivalent vaccine}
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention is a multivalent immunogenic composition for immunizing an animal against filariasis. In some embodiments, the antigens of the multivalent immunogenic composition are protein-based, DNA-based, or a combination thereof. This invention also provides a method and kit for detecting a filarial nematode and determining vaccine efficacy.

## Claims

### Claim 1

A multivalent immunogenic composition comprising an Abundant Larval Transcript antigen comprising SEQ ID NO: 122, a Small heat shock protein (HSP) 12.6 antigen comprising SEQ ID NO:81, and a Thioredoxin Peroxidase 2 antigen comprising SEQ ID NO: 83, in combination with two or more adjuvants.

### Claim 2 (depends on 1)

The multivalent immunogenic composition of claim 1, wherein the Abundant Larval Transcript comprises SEQ ID NO: 79 or SEQ ID NO: 99.

### Claim 3 (depends on 1)

The multivalent immunogenic composition of claim 1, wherein the Abundant Larval Transcript comprises SEQ ID NO: 93; the Small heat shock protein 12.6 comprises SEQ ID NO: 91; and the Thioredoxin Peroxidase 2 comprises SEQ ID NO: 95, or fragments thereof.

### Claim 4 (depends on 1)

The multivalent immunogenic composition of claim 1, wherein the antigens are covalently attached.

### Claim 5 (depends on 1)

A recombinant vector comprising nucleic acids encoding the multivalent immunogenic composition of claim 1.

### Claim 6 (depends on 5)

A recombinant host cell comprising the recombinant vector of claim 5.

### Claim 7 (depends on 1)

A method for inducing a protective immune response in a subject comprising administering the multivalent immunogenic composition of claim 1 to a subject thereby inducing a protective immune response in the subject.

### Claim 8 (depends on 7)

The method of claim 7, further comprising administering one or more additional doses of the multivalent immunogenic composition to the subject.

### Claim 9 (depends on 7)

The method of claim 7, wherein the immunogenic composition is administered by subcutaneous or intramuscular injection.

### Claim 10

A method for immunizing an animal against dirofilariasis comprising administering to a subject a multivalent immunogenic composition comprising two or more antigens, and in combination with two or more adjuvants, wherein said antigens are selected from an Abundant Larval Transcript antigen comprising SEQ ID NO: 121 or SEQ ID NO:122; a Small heat shock protein 12.6 antigen comprising SEQ ID NO: 81 or SEQ ID NO: 123; a Tetraspanin antigen comprising SEQ ID NO: 82; and a Thioredoxin Peroxidase 2 antigen comprising SEQ ID NO: 83 or SEQ ID NO:124 thereby immunizing the subject against dirofilariasis.

### Claim 11 (depends on 10)

The method of claim 10, further comprising administering one or more additional doses of the multivalent immunogenic composition to the subject.

### Claim 12 (depends on 10)

The method of claim 10, wherein the immunogenic composition is administered by subcutaneous or intramuscular injection.