Extracellular vesicles derived from recombinant microorganism including polynucleotide encoding target protein and use thereof

An extracellular vesicle derived from a recombinant microorganism comprising one or more polynucleotides encoding one or more target proteins, wherein the microorganism is a yeast, wherein the extracellular vesicle is an isolated extracellular vesicle, wherein the target protein is linked to a signal peptide, thereby making the microorganism load the target protein into the extracellular vesicle in an increased amount than the target protein not linked to the signal peptide and wherein the signal peptide is encoded by the nucleotide sequence of SEQ ID NO:4 and the extracellular vesicle comprises the target protein.