Materials and methods for performing histochemical assays for human pro-epiregulin and amphiregulin
AssigneeVentana Medical Systems, Inc.
InventorsFernando Jose Rebelo do Couto, Zhiming Liao, Andrea Muranyi, Kandavel Shanmugam, Shalini Singh, Yifei Zhu
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Abstract
The invention provides anti-human pro-epiregulin and anti-human amphiregulin antibodies and methods of using the same. Anti-EREG antibodies raised against amino acids 148-169 and 156-169 of the human EREG protein, and anti-AREG antibodies raised against amino acids 238-252 of the human AREG protein are disclosed. Methods of using these antibodies to detect EREG and AREG and kits and other products for performing such methods are also disclosed.
Claims25
An antibody or antibody fragment capable of specifically binding to amino acids 148-169 of SEQ ID NO:
- 1, wherein the antibody or antibody fragment comprises a heavy chain and a light chain, wherein the heavy chain comprises the following hypervariable regions (HVRs):
- (a) an HVR-H1 comprising SEQ ID NO:
- 2;
- (b) an HVR-H2 comprising SEQ ID NO:
- 3;
- and
- (c) an HVR-H3 comprising SEQ ID NO:
- 4;
- and
- wherein the light chain comprises the following HVRs:
- (h) an HVR-L1 comprising SEQ ID NO:
- 9;
- (i) an HVR-L2 comprising SEQ ID NO:
- 10;
- and
- (j) an HVR-L3 comprising SEQ ID NO:
- 11.
An antibody or antibody fragment capable of specifically binding to amino acids 148-169 of SEQ ID NO:
- 1, wherein the antibody or antibody fragment retains the ability to bind to human pro-epiregulin, and wherein the antibody or antibody fragment comprises a heavy chain variable domain (VH) comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 16 and a light chain variable domain (VL) sequence comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 17;
- wherein the VH comprises the HVRs:
- (a) HVR-H1 comprising SEQ ID NO:
- 2, (b) HVR-H2 comprising SEQ ID NO:
- 3, and (c) HVR-H3 comprising SEQ ID NO:
- 4;
- and wherein the VL comprises the HVRs:
- (a) HVR-L1 comprising SEQ ID NO:
- 9;
- (b) HVR-L2 comprising SEQ ID NO:
- 10;
- and (c) HVR-L3 comprising SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.