Hyperpolarization micro-magnetic resonance imager and three-dimensional imaging of a biological composition with cellular resolution

A hyperpolarization micro-magnetic resonance imager for three-dimensional imaging of a biological composition with cellular resolution, the hyperpolarization micro-magnetic resonance imager comprising:

• a hyperpolarizer source in electrical communication with a hyperpolarizer transmission line and in electrical communication with an NMR console and in electrical communication with an integrated imaging cell, hyperpolarizer, bioreactor and that receives a hyperpolarizer control signal from the NMR console, produces a hyperpolarizer microwave signal from the hyperpolarizer control signal, and communicates the hyperpolarizer microwave signal to the integrated imaging cell, hyperpolarizer, bioreactor via a hyperpolarizer transmission line;
• the hyperpolarizer transmission line disposed on a printed circuit board with integrated shield and in electrical communication with a hyperpolarizer source and in electrical communication with the integrated imaging cell and that receives the hyperpolarizer microwave signal from the hyperpolarizer source and communicates the hyperpolarizer microwave signal to the integrated imaging cell, hyperpolarizer, bioreactor;
• a nuclear magnetic resonance tuned circuit disposed on the printed circuit board with integrated shield and in electrical communication with a transcoupler and in electrical communication with the integrated imaging cell and that is capacitively coupled to the transcoupler and a nuclear magnetic resonance coil of the integrated imaging cell, hyperpolarizer, bioreactor, and tunes the nuclear magnetic resonance coil to resonate at the same frequency as the proton precession frequency of protons in the imaging cell, and provides an impedance so that a maximum RF power is delivered to a biological composition disposed in the imaging cell;
• a magnet disposed on the printed circuit board with integrated shield and in electrical communication with a magnet supply and in magnetic communication with the imaging cell and that receives the magnetic field modulation signal from the magnet supply, produces a magnetic field for performing magnetic resonance imaging of the biological composition in the imaging cell, and communicates the magnetic field to the imaging cell;
• a gradient-shim coil disposed on the printed circuit board with integrated shield and in magnetic communication with the magnet and in magnetic communication with the imaging cell and that is interposed between the magnet and the imaging cell and that increases homogeneity of the magnetic field and provides a magnetic field gradient for nuclear magnetic resonance imaging;
• a Hall probe and thermometer disposed on the printed circuit board with integrated shield and in electrical communication with a digital control system and that measure the magnetic field and temperature of the imaging cell, produces a magnetic sensor signal that indicates the temperature of the imaging cell and strength of the magnetic field, and communicates the magnetic sensor signal to the digital control system;
• the printed circuit board with integrated shield in mechanical communication with the hyperpolarizer transmission line and in mechanical communication with the integrated imaging cell and in mechanical communication with the hyperpolarizer and in mechanical communication with the bioreactor, such that the hyperpolarizer transmission line, the integrated imaging cell, the hyperpolarizer, bioreactor, and the nuclear magnetic resonance tuned circuit are disposed and arranged on the printed circuit board with integrated shield, such that the integrated imaging cell, hyperpolarizer, bioreactor and the Hall probe and thermometer are interposed between a pair of the magnets and a pair of the gradient-shim coils;
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• the integrated imaging cell, hyperpolarizer, bioreactor disposed on the printed circuit board with integrated shield and disposed on the hyperpolarizer transmission line and in mechanical communication with the printed circuit board with integrated shield and in mechanical communication with the hyperpolarizer transmission line and that comprises a hyperpolarizer film stack, the imaging cell, and the nuclear magnetic resonance coil.