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US 12,416,639 B2Granted
Reagents and methods for detecting protein lysine lactylation
AssigneeJingjie PTM BioLab (Hangzhou) Co., Ltd.
InventorsYingming Zhao, Di Zhang, Lunzhi Dai
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Abstract
The invention provides an isolated peptide comprising a lactylated lysine and a specific affinity reagent that specifically binds to a lactylated lysine in a peptide. Also provided are a method for detecting a lactylated lysine in a protein or a fragment thereof using the affinity reagent and a method for isolating the affinity reagent.
Claims6
A method for detecting a lactylated lysine in a protein or a fragment thereof, comprising:
- (a) contacting the protein or a fragment thereof with an affinity reagent selected from the group consisting of pan anti-Kla (PTM-1401) antibody, anti-H3K18la (PTM-1406) antibody, and anti-H4K5la (PTM-1407) antibody, whereby a binding complex of the protein or a fragment thereof and the affinity reagent is formed, and
- (b) detecting the binding complex, wherein the presence of the binding complex indicates the presence of a lactylated lysine in the protein or a fragment thereof.
Dependencies are inferred from the claim text.