Methods for labeling amine-containing compounds
AssigneeAgilent Technologies, Inc.
InventorsMichael J. Kimzey, Alexander Gyenes
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Abstract
The invention relates to an activated form of procaine, and the use of the activated procaine, or salts or solvates thereof, to label amine-containing compounds, such as N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in a sample. Use of activated procaine as a label allows for sensitive detection of compounds labeled with it by both fluorescence and mass spectrometry.
Claims19
A method for labeling and, optionally, analyzing, a N-glycan, amine-containing amino acid, amine-containing peptide, amine-containing protein, glycosylamine, or other amine-containing compound in a sample, said method comprising:
- (a) mixing said N-glycan, amine-containing amino acid, amine-containing peptide, amine-containing protein, glycosylamine, or other amine-containing compound in a solution with a labeling compound (“Activated Procaine”) with the structure
- or a solvate or a salt of said labeling compound, thereby creating a labeling mixture, and
- (b) conducting said labeling at a labeling temperature of between 10° C.±2° C. to 60° C.±2° C.,
- thereby creating a labeled, fluorescent, mass spectrometry-active derivative of said N-glycan, of said amine-containing amino acid, of said amine-containing peptide, of said amine-containing protein, of said glycosylamine, or of said other amine-containing compound in said sample.
Dependencies are inferred from the claim text.