Hollow polymer fiber optic system for single analyte and multiplexed analyte detection
AssigneeRevvity Health Sciences, Inc.
InventorsRoger Bossé, Wael I. Yared, Peter A. Harvey, Kevin Groves, Ilias Faqir, Michael Meltzer
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Abstract
Presented herein are methods, systems, and apparatus for single analyte detection or multiplexed analyte detection based on amplified luminescent proximity homogeneous assay (“alpha”) technology, but using hollow polymer fiber optics doped with ‘acceptor bead’ dye (e.g., thioxene, anthracene, rubrene, and/or lanthanide chelates) or ‘donor bead’ dye (e.g., phthalocyanine) that carry a signal generated by the dopant via singlet oxygen channeling.
Claims20
A hollow polymer optic fiber (i) doped with an acceptor dye composition that accepts singlet oxygen and as a consequence emits light and (ii) having at least one fiber binding partner that binds to an analyte of interest conjugated to its interior surface, the hollow polymer optic fiber capable of transmitting light generated by singlet oxygen channeling for the detection and/or quantification of the analyte of interest in a sample.
A bundle of hollow polymer optic fibers, each fiber of the bundle (i) doped with a corresponding acceptor dye composition that accepts singlet oxygen and as a consequence emits light and/or donor dye composition that releases singlet oxygen when illuminated with excitation light, and (ii) having at least one fiber binding partner that binds to an analyte of interest conjugated to its interior surface.
A hollow polymer optic fiber (i) doped with a donor dye composition that releases singlet oxygen when illuminated with excitation light and (ii) having at least one fiber binding partner that binds to an analyte of interest conjugated to its interior surface, the hollow polymer optic fiber capable of transmitting light generated by singlet oxygen channeling for the detection and/or quantification of the analyte of interest in a sample.
Dependencies are inferred from the claim text.