Analysis assistance device, method, and program for an analysis used in liquid chromatography

An analysis assistance method comprising:

• analyzing with an analysis device a sample containing a first substance, a second substance, and a third substance under a certain analysis condition;
• generating with a processor a chromatogram using data obtained from the analysis device;
• calculating with the processor an area percentage of a first peak in a case where the first peak, a second peak, and a third peak are included in the generated chromatogram, wherein:
• the area percentage of the first peak is a ratio of an area of the first peak to a sum of areas of the first to third peaks;
• and
• the first peak, the second peak, and the third peak correspond to the first substance, the second substance, and the third substance, respectively;
• determining with the processor a first resolution of the first peak and the second peak from a first retention time of the first peak and a second retention time of the second peak, and a first peak width of the first peak and a second peak width of the second peak;
• determining with the processor a second resolution of the first peak and the third peak from the first retention time and the third retention time of the third peak, and the first peak width and a third peak width of the third peak;
• and
• displaying with a display the chromatogram, such that:
• when the first resolution is not more than a predetermined resolution threshold and the second resolution is more than the predetermined resolution threshold and when the area percentage of the first peak is not less than a predetermined a area percentage threshold, the first peak and the second peak are both illustrated in a first displaying manner and the third peak is illustrated in a second displaying manner which is different from the first displaying manner.

A non-transitory computer readable medium storing an analysis assistance program causing a computer to execute:

• processing of generating a chromatogram using data obtained from an analysis device, wherein the analysis device analyzes a sample containing a first substance, a second substance, and a third substance under a certain analysis condition;
• processing of calculating an area percentage of a first peak in a case where the first peak, a second peak, and a third peak are included in the generated chromatogram, wherein:
• the area percentage of the first peak is a ratio of an area of the first peak to a sum of areas of the first to third peaks;
• and
• the first peak, the second peak, and the third peak correspond to the first substance, the second substance, and the third substance, respectively;
• and
• processing of determining a first resolution of the first peak and the second peak from a first retention time of the first peak and a second retention time of the second peak, and a first peak width of the first peak and a second peak width of the second peak;
• processing of determining a second resolution of the first peak and the third peak from the first retention time and the third retention time of the third peak, and the first peak width and a third peak width of the third peak;
• and
• processing of displaying on the display the chromatogram, such that:
• when the first resolution is not more than a predetermined resolution threshold and the second resolution is more than the predetermined resolution threshold and when the area percentage of the first peak is not less than a predetermined area percentage threshold, the first and second peaks are both illustrated in a first displaying manner and the third peak is illustrated in a second displaying manner which is different from the first displaying manner.