Method for hydrodynamics-assisted multi-parameter analyte spectrometry

A method of hydrodynamics-assisted analyte spectrometry, which makes use of a transparent microcapillary resonating (TMR) device, which comprises a substrate;

• a transparent capillary tube with two ends, through which a fluid flows with one or more analytes in suspension;
• supports coupled to the ends of the capillary tube;
• a piezoelectric module, coupled to the substrate and making contact with the capillary tube, suited to produce vibrations in the capillary tube;
• a laser that emits a beam of light;
• and a photoreceptor suited to receive the beam of light passing through the capillary tube before reaching the photoreceptor;
• wherein the method comprises the steps of:
• actuation of the laser, which emits the beam of light that hits the capillary tube and is picked up by the photoreceptor,
• generation of a first signal by the photoreceptor and amplification and filtering of the first signal,
• division of the amplified and filtered first signal into a modulated component and an unmodulated component,
• processing of the unmodulated component of the amplified and filtered first signal, and obtaining an optical signal of a measurement of a reflectivity of the analytes at each moment in time,
• detection of a passage of the analytes through an area of the capillary tube illuminated by the laser, and obtaining points of passage of ends of the analytes through a centre of the illuminated area, through the measurement of the reflectivity, for obtaining a time difference ΔT between the analyte entry and exit points to the illuminated area, and
• processing of the modulated component and obtaining a mechanical reference signal of a change in a resonance frequency of the analytes over time as the analytes flow through a free region of the capillary tube and obtaining a transit time (Tt) of the analytes through the capillary tube.