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AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsWilliam J. Greenleaf, Curtis J. Layton
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A novel method for displaying proteins and peptides is disclosed in which individual proteins or peptides remain associated with the DNA encoding them. Proteins or peptides can be generated by in vitro translation of DNA templates, either free in solution or arrayed on a solid support, such that the proteins or peptides remain immobilized on their DNA templates. In particular, high throughput sequencing can be combined with high throughput functional characterization of encoded proteins and peptides, wherein the identity of each protein or peptide is determined by DNA sequencing, and functional studies are carried out directly on each protein or peptide while immobilized on the DNA template encoding it. The methods of the invention should find numerous applications, for example, in high throughput genetic or pharmacological screening, epitope mapping, and protein engineering and directed evolution.
A composition comprising a deoxyribonucleic acid (DNA) molecule attached to a surface, said DNA molecule comprising a ribosome stall sequence, wherein said ribosome stall sequence comprises (i) a polyproline-encoding sequence adjacent to a stop codon, wherein said polyproline sequence is located on a 5′ side of said stop codon, and (ii) an arginine-histidine-arginine tricoding sequence adjacent to said polyproline-coding sequence.
Dependencies are inferred from the claim text.