Identification of an α-1,2-fucosyltransferase for the in vivo production of pure LNFP-I
AssigneeDsm IP Assets B.V.
InventorManos Papadakis
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Abstract
The present disclosure discloses the identification and introduction of a specific heterologous gene (denoted as smob), which encodes an α-1,2-fucosyltransferase, into an LNT production strain to produce LNFP-I in particular. The smob gene originates from the organism Sulfuriflexus mobilis (https://www.dsmz.de/collection/catalogue/details/culture/DSM-102939), which is a sulfur-oxidizing bacterium isolated from a brackish lake sediment.
Claims19
A genetically engineered cell capable of producing lacto-N-fucopentaose I (LNFP-I) comprising a recombinant nucleic acid sequence encoding an α-1,2-fucosyltransferase protein comprising SEQ ID NO:
- 1, or a functional homologue thereof having an amino acid sequence which is at least 90% identical to SEQ ID NO:
- 1, wherein the α-1,2-fucosyl-transferase is capable of transferring of a fucosyl moiety from a donor molecule onto a galactose moiety of lacto-N-tetraose (LNT) through an α-1,2 coupling.
A nucleic acid construct comprising a recombinant nucleic acid sequence which is at least 85% identical to SEQ ID NO:
- 2, wherein the expression of the nucleic acid construct is regulated by a promoter sequence selected from the group consisting SEQ ID NO:
- 16-38 and 39.
Dependencies are inferred from the claim text.