Expression cassette containing overlapping open reading frames and application

Abstract

An expression cassette containing overlapping open reading frames and an application thereof are provided. The overlapping open reading frames are overlapping open reading frames of a first ORF and a second ORF and include in sequence from a 5′ end to a 3′ end: a first promoter at least used to drive gene transcription of the first ORF; a 5′ part of a gene of the first ORF; an intron; and a 3′ part of a gene of the second ORF, the intron including a second promoter used only to drive gene transcription of the second ORF. By arranging two promoters in a single expression cassette in the disclosure, the two promoters are used to drive the expression of proteins of the overlapping reading frames and regulate the relative expression time and expression intensity of different proteins.

Dependencies are inferred from the claim text.