Expression of nitrogenase polypeptides in plant cells
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsRobert Silas Allen, Maria Christina Gregg, Shoko Okada, Amratha Menon, Charles Andrew Warden, Matthew Craig Taylor, Craig Christopher Wood
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Abstract
The present invention relates to methods and means for producing nitrogenase polypeptides in the mitochondria of plant cells.
Claims17
A plant cell comprising an exogenous polynucleotide which encodes a NifD fusion polypeptide which comprises a mitochondrial targeting peptide (MTP) translationally fused to a NifD polypeptide which comprises an amino acid sequence other than RRNY (SEQ ID NO:
- 101), RANY (amino acids 4-7 of SEQ ID NO:
- 85), and RRHY (amino acids 4-7 of SEQ ID NO:
- 86), at positions corresponding to amino acids 97-100 of SEQ ID NO:
- 18, wherein the exogenous polynucleotide comprises a promoter which is operably linked to a nucleotide sequence which encodes the NifD fusion polypeptide and which expresses said nucleotide sequence in the plant cell.
A NifD fusion polypeptide comprising a mitochondrial targeting peptide (MTP) translationally fused to a NifD polypeptide (ND), or a cleaved product thereof which comprises a scar sequence and the ND, wherein the NifD fusion polypeptide or the cleaved product thereof comprises an amino acid sequence other than RRNY (SEQ ID NO:
- 101) at positions corresponding to amino acids 97-100 of SEQ ID NO:
- 18.
Dependencies are inferred from the claim text.