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AssigneeFlagship Pioneering Innovations VI, LLC
InventorsMaria Helena Christine Van Rooijen, Hok Hei Tam, Maier Steve Avendaño Amado, Barry Andrew Martin, Ignacio Martinez, Piotr Stanislaw Kowalski, Nataliya Vladimirovna Nukolova, Yajie Niu
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Disclosed herein are plant-modifying compositions including a plurality of plant messenger packs (PMPs) (e.g., including a plant extracellular vesicle (EV), or segment, portion, or extract thereof), wherein the PMPs include a plant-modifying agent, and wherein the plant-modifying agent comprising the heterologous RNA. The compositions herein are useful in methods for modifying plants such as to increase plant fitness, wherein the increase in plant fitness comprising an increase in development, growth, yield, resistance to abiotic stressors, or resistance to biotic stressors.
A method of administering at least one heterologous RNA to an intact plant, the method comprising non-viral delivery to the intact plant of a composition comprising a plurality of lipid-reconstituted plant messenger packs (LPMPs), each of the plurality comprising the at least one heterologous RNA, an exogenous cationic lipid, and at least one plant protein extracellular vesicle marker, wherein the exogenous cationic lipid increases the RNA loading efficiency as compared to the loading efficiency of an LPMP not comprising the at least one heterologous cationic lipid, and wherein the composition is delivered at an effective concentration to increase plant fitness.
Dependencies are inferred from the claim text.