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US 12,415,991 B2Granted
Recombinant heme thiolate oxygenases
AssigneeBisy GmbH
InventorsKay Domenico Novak, Anton Glieder, Astrid Weninger, Christoph Reisinger, Claudia Rinnofner, Carsten Pichler
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Abstract
The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides. The invention also relates to improved methods of producing such polypeptides in yeasts.
Claims7
A modified unspecific peroxygenase (UPO) comprising an amino acid sequence having at least 75% sequence identity to the polypeptide of SEQ ID NO:
- 12 and having increased peroxygenase activity as compared to unmodified wild-type UPO12, which is the polypeptide of SEQ ID NO:12, wherein the modification is a modification of at least one amino acid selected from D145, E249, D253, and/or C256 of the polypeptide of SEQ ID NO:12, and wherein the peroxygenase activity is increased by 1.3-fold or more when measured in an ABTS assay or a 2,6-DMP assay.
A modified unspecific peroxygenase (UPO) having increased peroxygenase activity as compared to the unmodified wild-type UPO12 of SEQ ID NO:
- 12, wherein the modified UPO comprises SEQ ID NO:30, or an amino acid sequence having at least 90% sequence identity to SEQ ID NO:30 and comprising an amino acid modification of the amino acid corresponding to S24 of SEQ ID NO:12.
Dependencies are inferred from the claim text.