HPLC free purification of peptides by the use of new capping and capture reagents
AssigneeRoche Diagnostics Operations, Inc.
InventorsFrank Bergmann, Sebastian Johannes Pomplun
Life of this patent
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Abstract
The present disclosure relates to the use of a capping and capture reagent in solid phase peptide synthesis. The present disclosure further relates to a method of solid phase peptide synthesis, wherein a capping and capture reagent according to the present disclosure is used. The present disclosure further relates to a method for purification of a (full-length) synthetic peptide via use of a capping and capture reagent according to the present disclosure. The present disclosure also relates to a kit comprising a capping and capture reagent according to the present disclosure and an amino oxy resin or a hydrazine resin and the use of the kit.
Claims4
A method for purification of a synthetic peptide comprising the steps of:
- performing a solid phase peptide synthesis (SPPS), wherein individual amino acids are coupled to each other via Fmoc-chemistry, wherein after at least one coupling step a capping and capture reagent is used for capping a failure peptide sequence, said capping and capture (cap-capt) reagent comprising a capping moiety and a capture moiety, and wherein said capping moiety is a carboxylic acid or an activated carboxylic acid and wherein said capture moiety is a protected carbonyl group, the capping and capture reagent being selected from
- and
- cleaving the compounds thus synthesized from the solid phase by adding trifluoroacetic acid (TFA) or TFA combined with one or more scavengers, wherein said one or more scavengers are selected from water, a silane, a thiol, a sulfide, and/or an ether,
- deprotecting the capture moiety using trifluoroacetic acid (TFA) or TFA combined with one or more scavengers, wherein said one or more scavengers are selected from water, a silane, a thiol, a sulfide, and/or an ether, and
- binding the deprotected capture moiety to a solid resin support thereby binding the at least one failure peptide sequence to the solid resin support and
- separating the synthetic peptide from the solid resin support comprising the failure peptide.
Dependencies are inferred from the claim text.