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US 12,415,017 B2Granted
3D printing bio gel and method of use
AssigneeTDBT IP Inc.
InventorsDaniel Cohen, Chris Pelletier
Life of this patent
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Abstract
Collagen compositions, methods for preparing those collagen compositions, and 3D constructs formed from those collagen compositions are provided. In particular, methods of isolating collagen that exhibits an enhanced rate of gelling, such collagen compositions, and 3D constructs formed from such collagen compositions are provided.
Claims20
A bio gel composition comprising:
- collagen material in an amount greater than 5 mg/mL of the total bio gel composition;
- and
- a weak acid solution;
- wherein the bio gel composition:
- undergoes gelation in less than about 3 minutes at a temperature of about 25° C. to about 37° C.;
- is liquid under shear stress of about 15 Pa to about 100 Pa;
- and
- remains solid during gelation under hydrostatic pressure of about 30 Pa to about 120 Pa;
- and
- wherein the bio gel composition is obtained by a method comprising:
- providing a first receptacle having the collagen in the weak acid solution;
- providing a second receptacle having a carrier;
- and
- contacting the collagen material in the weak acid solution of the first receptacle with the carrier of the second receptacle to obtain the bio gel composition, wherein the contacting comprises stirring the collagen material in the weak acid solution and the carrier to form the bio gel composition until the bio gel composition has a homogeneity of about 50% to about 99.9%;
- and
- wherein the collagen material is obtained by a method comprising:
- (a) processing a collagen-based biomaterial to obtain biomaterial particles;
- (b) contacting the biomaterial particles with a weak acid solution to obtain a collagen-containing solution;
- (c) segregating the collagen-containing solution to obtain an extracted collagen solution;
- (d) contacting the extracted collagen solution with a salt solution to obtain a collagen precipitate;
- (e) re-suspending the collagen precipitate in a weak acid solution to obtain a resuspended collagen solution;
- and performing viral and prion inactivation of the re-suspended collagen solution;
- (f) desalting the re-suspended collagen solution;
- and
- (g) drying the re-suspended collagen solution to obtain the collagen material.
22 more elements
A method of harvesting collagen for use in bio gel compositions, the method comprising:
- (a) processing a collagen-based biomaterial to obtain biomaterial particles;
- (b) contacting the biomaterial particles with a weak acid solution to obtain a collagen-containing solution;
- (c) segregating the collagen-containing solution to obtain an extracted collagen solution;
- (d) contacting the extracted collagen solution with a salt solution to obtain a collagen precipitate;
- (e) re-suspending the collagen precipitate in a weak acid solution to obtain a re-suspended collagen solution;
- and optionally, performing viral and prion inactivation of the re-suspended collagen solution;
- (f) desalting the re-suspended collagen solution;
- (g) drying the re-suspended collagen solution to obtain a collagen material;
- and
- (h) sterilizing the collagen material.
7 more elements
Dependencies are inferred from the claim text.