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US 12,414,999 B2Granted
Compositions and methods relating to macrophages and/or monocytes with adhered particles
AssigneesPresident and Fellows of Harvard College, The Regents of the University of California
InventorsSamir Mitragotri, Michael Andrew Evans, Charles Wyatt Shields, IV
Life of this patent
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Abstract
Provided herein are polymeric particles and compositions (i.e., “backpacks”) that can adhere to cells and provide delivery of payload immunomodulatory agents to those cells. For examples, the particles can adhere to macrophages and/or monocytes and release cytokines that promote an M1 or M2 phenotype to improve therapeutic efficacy of the cells.
Claims21
A polymeric particle comprising:
- a) a first layer comprising a face of the polymeric particle and:
- i) one or more cell adhesive molecules, the one or more cell adhesive molecules comprising one or more of cell adhesive polyelectrolytes, immunoglobulins, and/or ligands for receptors on monocyte or macrophage cell surfaces;
- and
- ii) at least one M1-polarizing agent or M2-polarizing agent;
- and
- b) a second layer comprising one or more structural polymers;
- wherein the diameter of the polymeric particle is from 100 nm to 1 μm.
4 more elements
An engineered cellular composition comprising:
- a) a monocyte or macrophage cell;
- and
- b) a polymeric particle comprising:
- i) a first layer comprising a face of the polymeric particle and:
- 1) one or more cell adhesive molecules, the one or more cell adhesive molecules comprising one or more of cell adhesive polyelectrolytes, immunoglobulins, and/or ligands for receptors on monocyte or macrophage cell surfaces;
- and
- 2) at least one M1-polarizing agent or M2-polarizing agent;
- and
- ii) a second layer comprising one or more structural polymers;
- wherein the particle is located on the cell surface of the monocyte or macrophage.
7 more elements
A polymeric particle comprising:
- at least one M1-polarizing agent or M2-polarizing agent;
- a first layer comprising one or more cell adhesive molecules on a face of the polymeric particle, the one or more cell adhesive molecules comprising one or more cell adhesive polyelectrolytes;
- and
- a second layer comprising one or more structural polymers, the second layer being formed by spin coating.
1 more element
Dependencies are inferred from the claim text.