Antigenically stealthed oncolytic viruses
AssigneesUniversity of Pittsburgh—Of the Commonwealth System of Higher Education, The Trustees of the University of Pennsylvania
InventorsJoseph C. Glorioso, III, Gary H. Cohen, Doina Atanasiu, Tina Marie Cairns, Bonnie Lynn Hall, Justus B. Cohen, Ceren Tuzmen
Life of this patent
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Abstract
Provided herein are antigenically stealthed HSV particles, and methods of use of the antigenically-stealthed HSV particles.
Claims21
A retargeted herpes simplex virus (HSV) particle, comprising a genome, a capsid, tegument, and an envelope comprising an antigenically-modified glycoprotein D (gD) protein, wherein one or more of amino acids 10-20, 54, 75-79, 132, 140, 213, 216, 222-224, and 262-279 of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, or one or more amino acids corresponding to amino acids 10-20, 54, 75-79, 132, 140, 213, 216, 222-224, and 262-279 of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, are modified in the gD glycoprotein of the virus particle, and
- wherein the modifications:
- reduce or eliminate neutralization of the retargeted virus particle by antibodies that recognize the gD glycoprotein, and
- permit gD glycoprotein-mediated infection of a target cell by the retargeted virus particle.
A retargeted herpes simplex virus (HSV) particle, comprising a genome, a capsid, tegument, and an envelope comprising an antigenically-modified glycoprotein D (gD) protein, wherein the antigenically-modified gD protein comprises a P54Q and/or a T213M substitution in SEQ ID NO:
- 1 or SEQ ID NO:
- 2 and optionally one or more of amino acids 10-20, 75-79, 132, 140, 216, 222-224, and 262-279 of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, or one or more amino acids corresponding to amino acids 10-20, 75-79, 132, 140, 216, 222-224, and 262-279 of SEQ ID NO:
- 1 or SEQ ID NO:
- 2, are modified in the gD glycoprotein of the virus particle, and
- wherein the modifications:
- reduce or eliminate neutralization of the retargeted virus particle by antibodies that recognize the gD glycoprotein, and
- permit infection of a target cell by the retargeted virus particle.
Dependencies are inferred from the claim text.