Back to results
US 12,411,131 B2Granted
Assay methods
AssigneeMeso Scale Technologies, LLC.
InventorsAnahit Aghvanyan, Eli N. Glezer, John Kenten, George Sigal, Martin Stengelin, David Routenberg
Life of this patent
Estimating the term…
Abstract
The present invention is directed to methods for improving assay specificity and performance in binding assays.
Claims21
A method of detecting an analyte of interest in a sample, comprising:
- (a) forming a complex on a surface, wherein the complex comprises (i) a capture reagent for the analyte that is bound to the surface;
- (ii) the analyte;
- and (iii) a detection reagent for the analyte that is linked to a nucleic acid probe, wherein the surface further comprises an anchoring reagent comprising an anchoring oligonucleotide;
- (b) extending the nucleic acid probe to form an extended sequence comprising an anchoring oligonucleotide complement that is complementary to the anchoring oligonucleotide;
- (c) hybridizing the anchoring oligonucleotide to the anchoring oligonucleotide complement;
- and
- (d) measuring the amount of extended sequence bound to the surface, thereby detecting the analyte of interest in the sample.
4 more elements
Dependencies are inferred from the claim text.