Apparatus and method for automated microdissection of tissue from slides to optimize tissue harvest from regions of interest

An apparatus for automated microdissection of tissue from slides to optimize tissue harvest from regions of interest, the apparatus comprising:

• at least a processor;
• and
• a memory communicatively connected to the at least a processor, wherein the memory contains instructions configuring the at least a processor to:
• receive a stained input slide;
• identify a region of interest on the stained input slide, wherein identifying the region of interest comprises using a registration module to:
• log a sample size from the stained input slide;
• document a plurality of slides sectioned for analysis;
• predict a size and a location of the region of interest as a function of the sample size and the number of slides;
• and
• register the identified region of interest across a plurality of unstained slides;
• generate a segmentation map of the region of interest as a function of a segmentation algorithm;
• and
• register a segmented region of interest, as a function of the segmentation map, onto an unstained slide, wherein registering the segmented region of interest onto the unstained slide comprises:
• determining an orientation of the unstained slide corresponding to the segmented region of interest of the stained input slide;
• recording the orientation of the unstained slide relative to a reference plane;
• and
• registering the segmented region of interest to the unstained slide.