Method for producing lysine from microorganism and for constructing microorganism
AssigneeTianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
InventorsJibin Sun, Jiuzhou Chen, Ping Zheng, Wenjuan Zhou, Xuan Guo, Tuo Shi, Jiao Liu, Yanhe Ma
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Abstract
Provided are Corynebacterium glutamicum that produces L-lysine, a method for constructing the L-lysine producing strain, and a method for producing L-lysine from the strain. The lysine yield and glucose conversion of the L-lysine producing strain are improved, thereby reducing the production cost.
Claims14
An L-lysine producing bacterium Corynebacterium glutamicum, wherein, compared to a wild-type bacterium containing an endogenous polypeptide, the L-lysine producing bacterium has at least one of the following characteristics (i) to (iii):
- (i) a reduced or eliminated polypeptide activity of the endogenous polypeptide;
- (ii) a reduced or eliminated expression level of a coding gene of the endogenous polypeptide;
- and
- (iii) a reduced or eliminated expression level of a polynucleotide of interest;
- wherein by having at least one of the characteristics (i) to (iii), L-lysine production in the L-lysine producing bacterium Corynebacterium glutamicum is increased as compared to that in the wild-type bacterium,
- wherein the endogenous polypeptide is a polypeptide having the amino acid sequence of SEQ ID NO:
- 1, or a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 1;
- and
- the polynucleotide of interest is a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 2, or a polynucleotide having at least 90% sequence identity to the nucleotide sequence of SEQ ID NO:
- 2.
A method for constructing an L-lysine producing bacterium Corynebacterium glutamicum, wherein the method comprises:
- modifying a wild-type bacterium containing an endogenous polypeptide to reduce or eliminate a polypeptide activity of the endogenous polypeptide of the wild-type bacterium;
- modifying a wild-type bacterium containing an endogenous polypeptide to reduce or eliminate an expression level of a coding gene of the endogenous polypeptide of the wild-type bacterium;
- or
- modifying a wild-type bacterium to reduce or eliminate an expression level of a polynucleotide of interest in the wild-type bacterium;
- wherein by modifying the wild-type bacterium, L-lysine production in the L-lysine producing bacterium Corynebacterium glutamicum is increased as compared to that in the wild-type bacterium,
- wherein the endogenous polypeptide is a polypeptide having the amino acid sequence of SEQ ID NO:
- 1, or a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
- 1;
- and
- the polynucleotide of interest is a polynucleotide having the nucleotide sequence of SEQ ID NO:
- 2, or a polynucleotide having at least 90% sequence identity to the nucleotide sequence of SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.