Compositions and methods for treating sensorineural hearing loss using otoferlin dual vector systems

A dual vector system comprising:

• a first nucleic acid vector comprising a myosin 15 (Myo15) promoter operably linked to a first coding polynucleotide that encodes an N-terminal portion of an otoferlin (OTOF) protein;
• and
• a second nucleic acid vector comprising a second coding polynucleotide that encodes a C-terminal portion of an OTOF protein and a polyadenylation (poly(A)) sequence positioned at a 3′ end of the second coding polynucleotide;
• wherein the Myo15 promoter has at least 95% sequence identity to SEQ ID NO:
• 38, wherein neither the first nor second nucleic acid vector encodes a full-length OTOF protein, and, when introduced into a mammalian cell, the first and second nucleic acid vectors undergo homologous recombination or concatemerization to form a recombined nucleic acid that encodes a full-length OTOF protein.