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US 12,410,436 B2Granted
Adeno-associated virus (AAV) producer cell lines
AssigneeLonza Houston, Inc.
InventorsBingnan Gu, Gang Li, Caitlin Tripp
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Abstract
The present disclosure relates to nucleic acids encoding helper genes and adeno-associated virus (AAV) genes, under the control of inducible promoters. The disclosure also relates to a mammalian cell line for producing AAV as well as methods of using the mammalian cells for producing AAVs.
Claims3
An isolated nucleic acid molecule operably comprising:
- a. a first transposon-specific inverted terminal repeat (ITR);
- b. a first inducible promoter comprising a native E2A promoter and two tetracycline operator sequences (a first TetO2 sequence);
- c. an E2A coding sequence under control of the first inducible promoter;
- d. a viral associated (VA) non-coding RNA under control of a second inducible promoter comprising a native VA promoter or a constitutive promoter comprising a native VA promoter;
- e. a third inducible promoter comprising a promoter native to an E4 coding sequence and a second TetO2 sequence;
- f. the E4 coding sequence under control of the third inducible promoter;
- g. an antibiotic resistance gene;
- and
- h. a second ITR,
- wherein the isolated nucleic acid molecule does not include a Rep gene and/or Cap gene.
7 more elements
Dependencies are inferred from the claim text.