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US 12,410,432 B2Granted
L-RNA aptamer cyclization and use thereof
AssigneeCity University of Hong Kong
InventorsChun Kit Kwok, Danyang Ji
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Abstract
A method of cyclizing an L-RNA aptamer by modifying the aptamer with a 3′ azide and a 5′ alkyne group and using click chemistry reaction-based method. The cyclized L-RNA aptamers have improved binding properties and favour more in vitro/cell applications. Also disclosed is an L-oligonucleotide aptamer having linked ends.
Claims7
A method of linking two terminal ends of oligonucleotide, wherein one of the terminal oligonucleotide ends has a 5′ terminal alkyne;
- and the other one of the terminal oligonucleotide ends has a 3′ azide residue;
- comprising the step of:
- linking the 5′ terminal alkyne to the 3′ azide residue,
- wherein the oligonucleotide is L-Apt.4-1c comprising the nucleotide sequence of SEQ ID NO:
- 1.
2 more elements
Dependencies are inferred from the claim text.