Methods for purification of messenger RNA

A composition comprising at least 10 grams of dried purified mRNA, wherein said mRNA is obtained by a method for large-scale purification of mRNA, comprising the steps of:

• providing a suspension comprising precipitated mRNA manufactured by in vitro synthesis;
• and
• centrifuging the suspension at a speed of about 1000 to 5000 RPM in a centrifuge comprising a porous substrate such that the precipitated mRNA is captured on the porous substrate, and contaminants are removed from the precipitated mRNA to provide captured purified mRNA;
• washing the captured purified mRNA composition with a solvent;
• and
• drying the captured purified mRNA, thereby obtaining dried purified mRNA in the form of a solid,
• wherein the purified mRNA yield is at least about 80% and is substantially free of prematurely aborted RNA sequences, DNA templates, and one or more enzyme reagents used in in vitro synthesis;
• wherein the dried purified mRNA is collected and stored as a solid at a temperature of or below about 0° C. for a period of at least about one week to about two years;
• and
• wherein the dried purified mRNA has substantially the same integrity as prior to storage.