Use of enzyme that cleaves both alpha- and beta- 1,4-glycosidic bonds
AssigneeKorea University Research and Business Foundation
InventorsKyoung Heon Kim, Do Hyoung Kim
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Abstract
The present invention relates to use of an enzyme that cleaves both alpha- and beta-1,4-glycosidic bonds and, more particularly, the present invention provides α-L-fucosidase which has α-1,4-glucosidase and β-1,4-galactosidase activities and thus is capable of producing glucose and/or galactose, which are monosaccharides, from non-fucosylated saccharides. The enzyme has transglycosylation activity and thus produces maltooligosaccharides having a higher degree of polymerization than maltooligosaccharides used as a substrate, thereby being applicable to the development of high-value-added new materials in the food industry.
Claims10
A composition for producing monosaccharides or maltooligosaccharides, comprising:
- α-L-fucosidase comprising an amino acid sequence of SEQ ID NO:
- 1;
- and
- one or more substrates selected from the group consisting of lactose and maltooligosaccharides having a degree of polymerization of 2 or higher,
- wherein the α-L-fucosidase is obtained from a host cell transformed with a recombinant vector comprising a gene encoding the α-L-fucosidase.
A method for producing monosaccharides or maltooligosaccharides, comprising:
- reacting one or more substrates selected from the group consisting of lactose and maltooligosaccharides having a degree of polymerization of 2 or higher with α-L-fucosidase comprising an amino acid sequence of SEQ ID NO:
- 1;
- and
- collecting monosaccharides and maltooligosaccharides from a reaction product, wherein the maltooligosaccharides have a higher degree of polymerization than that of maltooligosaccharides used as the substrate.
Dependencies are inferred from the claim text.