US 12,410,411 B2 · Biocatalytic techniques

Abstract

The present invention relates to a cytochrome P450 enzyme comprising the amino acid sequence set forth in SEQ ID NO: 3, or a variant thereof having an amino acid sequence having at least 95% identity thereto and having CYP450 activity. The cytochrome P450 enzyme provided herein was isolated from Streptomyces eurythermus NRRL 2539 and has a wide substrate range and high activity, and may be used to oxidate organic compounds.

Claims13

Dependent claims

A microorganism comprising a nucleic acid molecule comprising a nucleotide sequence encoding a cytochrome P450 enzyme comprising the amino acid sequence set forth in SEQ ID NO:

3, or a variant thereof having an amino acid sequence having at least 98% identity thereto and having CYP450 activity, wherein the nucleotide sequence encoding the enzyme is heterologous to the microorganism, or a lysate of said microorganism.

Dependencies are inferred from the claim text.

Facts

Application

17/778,315Filed 23 Nov 2020

Priority

GB 191707722 Nov 2019

Published

US 2023/0040326 A19 Feb 2023

Granted

9 Sep 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification18 codes

C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12N 15/70
Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression: vectors or expression systems…
C12P 7/26
Containing a carbonyl group: ketones
C12P 7/6409
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group;…:…

Biocatalytic techniques

Life of this patent

Biocatalytic techniques

Life of this patent

Abstract

Claims13