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US 12,410,410 B2Granted
Ketoreductase polypeptides for the synthesis of chiral compounds
AssigneeCodexis, Inc.
InventorsOscar Alvizo, Nicholas J. Agard, Xinkai Xie, David Entwistle, Birgit Kosjek
Life of this patent
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Abstract
The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.
Claims6
An engineered polypeptide comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:
- 240, wherein the residue corresponding to X150 is a tryptophan, the residue corresponding to X173 is a non-polar or aliphatic residue, the residue corresponding to X211 is an aromatic, acidic, or polar residue, and wherein the residue corresponding to X249 is glycine and wherein said polypeptide has ketoreductase activity with at least 2-fold greater selectivity to the chiral compound 2a relative to chiral compound 2c as compared to SEQ ID NO:2.
Dependencies are inferred from the claim text.