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US 12,410,392 B2Granted
Delivery of biomolecules to immune cells
AssigneesMassachusetts Institute of Technology, President and Fellows of Harvard College
InventorsArmon R. Sharei, Shirley Mao, George Hartoularos, Sophia Liu, Megan Heimann, Pamela Basto, Gregory Szeto, Siddharth Jhunjhunwala, Darrell J. Irvine, Robert S. Langer, Klavs F. Jensen, Ulrich H. Von Andrian
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Abstract
A method and device for preferentially delivering a compound such as an antigen to the cytosol of an immune cell. The method comprises passing a cell suspension comprising the target immune cell through a microfluidic device and contacting the suspension with the compound(s) or payload to be delivered.
Claims1
A method of treating a patient, comprising:
- (a) modifying immune cells to exhibit an immune function comprising a phenotype that is not naturally exhibited by the immune cells, wherein the immune function is capable of aiding in treating the patient, wherein the modifying comprises (i) passing a cell suspension comprising the immune cells through a microfluidic device and (ii) contacting the cell suspension with a compound such that the compound is delivered to the cytosol of the immune cells, wherein the compound is capable of conferring the immune function to the immune cells when delivered to the cytosol of the immune cells, wherein the microfluidic device comprises a constriction having a diameter of 2 μm to 10 μm;
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- (b) introducing the modified immune cells to the patient,
- wherein the immune function comprises an antigen presenting phenotype, wherein the immune cells do not naturally comprise an antigen presenting phenotype, wherein the immune cells that are modified are T cells, and wherein the compound is an antigen.
1 more element
Dependencies are inferred from the claim text.