Methods for purifying proteins having a tubulin carboxypeptidase activity and peptidic based inhibitors thereof

A method of treatment of a disorder involving altered microtubule detyrosination in a subject in need thereof comprising administering to the subject in need thereof an amino-acid or peptidic based inhibitor that inhibits, at least partially, a tubulin carboxypeptidase activity, wherein the amino acid or peptidic based inhibitor is selected from the group consisting of:

• a Tyr amino-acid (Y) modified with an epoxysuccinyl group, or a peptidic moiety consisting of an amino acid sequence selected from the group consisting of EDY, and EEY, where the tyrosine residue is chemically modified with an epoxysuccinyl group;
• a peptidic moiety of 2 to 19 amino acids of the most C-terminal amino acids of SEQ ID NO:12 where the C-terminal position is a tyrosine residue chemically modified with an epoxysuccinyl group;
• and
• a peptidic moiety consisting of an amino acid sequence selected from the group consisting of SEQ ID NO:
• 12, SEQ ID NO:13, SEQ ID NO:
• 14, SEQ ID NO:
• 15, SEQ ID NO:17, and SEQ ID NO:
• 18, where the C-terminal position is a tyrosine residue chemically modified with an epoxysuccinyl group.