Insulin analogs
AssigneesUniversity of Utah Research Foundation, The Walter and Eliza Hall Institute of Medical Research
InventorsJohn Gerbrandt Tasman Menting, Brian Smith, Danny Hung-Chieh Chou, Helena Safavi-Hemami, Michael Colin Lawrence, Baldomero M. Olivera
Life of this patent
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Abstract
The present invention relates to insulin analogs, particularly insulin analogs having shortened B chains. The present invention also relates to the crystal structure of insulin from the venom of cone snails and to methods of using the crystal and related structural information to screen for and design insulin analogs that interact with or modulate the insulin receptor. The present invention also relates to therapeutic and prophylactic methods using insulin analogs.
Claims18
An insulin analog comprising a human insulin A chain peptide at least 85% identical to wild-type human insulin A chain provided as SEQ ID NO:
- 24, and a human insulin B chain peptide comprising 90% identity to amino acids 1-22 of wild-type human insulin B chain provided as SEQ ID NO:25 and lacking the last 8 amino acids of the C-terminus of wild-type human insulin B chain, wherein the human insulin B chain peptide comprises a substitution at amino acid 10 and amino acid 20, wherein the substitution at amino acid 20 is G20Y, G20F, or G20P, and wherein the substitution at amino acid 10 is H10E, H10D or H10Q.
A therapeutic protein having a human insulin A chain peptide bonded to a human insulin B chain peptide via at least one disulfide bond, wherein the human insulin A chain comprises the sequence of GIVEQCCHRICSLYQLENYCN (SEQ ID NO:39), and wherein the human insulin B chain peptide comprises the sequence of FVNQHLCGSELVEALYLVCYER (SEQ ID NO:
- 30).
A therapeutic protein having a human insulin A chain peptide bonded to a human insulin B chain peptide via at least one disulfide bond, wherein the human insulin A chain comprises the sequence of GIVEQCCHRICSLYQLENYCG (SEQ ID NO:58), and wherein the human insulin B chain peptide comprises the sequence of FVNQHLCGSELVEALYLVCYER (SEQ ID NO:
- 30).
Dependencies are inferred from the claim text.