Oligosaccharide compositions and methods of use thereof for reducing ammonia levels
AssigneeDSM Nutritional Products AG
InventorsChristopher Matthew Liu, John M. Geremia, Anastasia Lioubomirov, Max Hecht
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Abstract
Aspects of the disclosure relate to oligosaccharide compositions and methods of making the same. Also provided are methods of using oligosaccharide compositions as microbiome metabolic therapies for reducing ammonia levels and for the treatment of hyperammonemia-related diseases (e.g., urea cycle disorders and hepatic encephalopathy).
Claims15
An oligosaccharide composition comprising a plurality of oligosaccharides, the plurality of oligosaccharides being characterized by a multiplicity-edited gradient-enhanced 1H-13C heteronuclear single quantum coherence (HSQC) NMR spectrum comprising signals 1, 3, and 4, each signal having properties as follows:
- Area under the curve (AUC)
- Center Position
- (% of total areas of signals observed
- (ppm)
- and assigned to the oligosaccharide
- Glycosidic
- Signal
- 1H
- 13C
- composition)
- Bond Type
- 1
- 5.39
- 100.31
- 3.37-4.78
- alpha-1,4
- 3
- 5.35
- 99.77
- 6.64-8.71
- alpha-1,3
- 4
- 5.22
- 102.55
- 0.00-0.95
- —.
An oligosaccharide composition comprising a plurality of oligosaccharides, each oligosaccharide comprising a plurality of monomer radicals;
- the plurality of oligosaccharides comprising two or more types of monomer radicals selected from radicals (1)-(7), wherein the selected radicals are present in the plurality of oligosaccharides in a defined molar percentage (mol %) range:
- (1) 2-glucopyranose diradicals, representing 6.15-9.54 mol % of monomer radicals in the plurality of oligosaccharides;
- (2) 6-glucopyranose diradicals, representing at least 18.94 mol % of monomer radicals in the plurality of oligosaccharides;
- (3) 4-glucopyranose diradicals, representing 6.63-8.74 mol % of monomer radicals in the plurality of oligosaccharides;
- (4) 3,4-glucopyranose triradicals, representing less than 1.42 mol % of monomer radicals in the plurality of oligosaccharides;
- (5) 2,3-glucopyranose triradicals, representing 1.01-2.59 mol % of monomer radicals in the plurality of oligosaccharides;
- (6) 3,4,6-glucopyranose tetraradicals, representing less than 1.00 mol % of monomer radicals in the plurality of oligosaccharides;
- and
- (7) 2,4,6-glucopyranose tetraradicals, representing less than 0.72 mol % of monomer radicals in the plurality of oligosaccharides;
- wherein at least one of the two or more types of monomer radicals is (4) or (7).
Dependencies are inferred from the claim text.