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US 12,409,449 B2Granted
Flow cells
AssigneeIllumina, Inc.
InventorsYir-Shyuan Wu, Tarun Kumar Khurana, Yasaman Farshchi, Xi-Jun Chen, Bernard Hirschbein
Life of this patent
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Abstract
An example of a flow cell includes a substrate and a cationic polymeric hydrogel on the substrate. The cationic polymeric hydrogel includes a cationic moiety that is i) integrated into a monomeric unit of an initial polymeric hydrogel or ii) attached to the monomeric unit of the initial polymeric hydrogel through a linker. The flow cell further includes an amplification primer attached to the cationic polymeric hydrogel.
Claims21
A flow cell, comprising:
- a substrate;
- a flow lane defined in the substrate;
- a cationic polymeric hydrogel on the substrate in at least a portion of the flow lane, the cationic polymeric hydrogel including a cationic moiety i) integrated into a monomeric unit of an initial polymeric hydrogel or ii) attached to the monomeric unit of the initial polymeric hydrogel through a linker;
- an amplification primer attached to the cationic polymeric hydrogel;
- a lid or second substrate bonded to the substrate;
- an input port in fluid communication with the flow lane;
- and
- an exit port in fluid communication with the flow lane.
5 more elements
A method, comprising:
- introducing a fluid, including a positively chargeable moiety, to a flow cell including:
- a substrate;
- a flow lane defined in the substrate;
- an initial polymeric hydrogel on the substrate in at least a portion of the flow lane, the initial polymeric hydrogel having a surface moiety selected from the group consisting of a negatively chargeable atom, an azide group, and an alkyne group;
- an amplification primer attached to the initial polymeric hydrogel;
- a lid or second substrate bonded to the substrate;
- an input port in fluid communication with the flow lane;
- and
- an exit port in fluid communication with the flow lane;
- and
- incubating the initial polymeric hydrogel in the fluid at a temperature and for a time, thereby forming a cationic polymeric hydrogel on the substrate in the at least the portion of the flow lane, the cationic polymeric hydrogel including a cationic moiety.
8 more elements
Dependencies are inferred from the claim text.