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US 12,409,195 B2Granted
Bacteria engineered to reduce hyperphenylalaninemia
AssigneeSynlogic Operating Company, Inc.
InventorsDean Falb, Vincent M. Isabella, Jonathan W. Kotula, Paul F. Miller, Yves Millet, Sarah Rowe
Life of this patent
Estimating the term…
Abstract
Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of modulating and treating diseases associated with hyperphenylalaninemia are disclosed.
Claims9
A nucleic acid sequence comprising one or more gene(s) encoding a phenylalanine ammonia lyase (PAL) and one or more gene(s) encoding a phenylalanine transporter,
- wherein the PAL is from Anabaena variabilis (PAL1) or Photorhabdus luminescens (PAL3), wherein the one or more genes encoding the PAL is operably linked to an inducible promoter that is not in nature associated with the one or more genes encoding the PAL, wherein the inducible promoter is induced by low oxygen or anaerobic conditions to enable expression of the PAL in an Escherichia probiotic bacterium;
- wherein the one or more genes encoding the phenylalanine transporter are from Acinetobacter calcoaceticus, Salmonella enterica, or Escherichia coli;
- and wherein the one or more genes encoding the phenylalanine transporter is operably linked to an inducible promoter that is not in nature associated with the one or more genes encoding the phenylalanine transporter, wherein the inducible promoter is induced by low oxygen or anaerobic conditions to enable expression of the phenylalanine transporter in the bacterium.
Dependencies are inferred from the claim text.