Traditional Chinese medicine composition and use thereof

A method for treating antipyretic, inflammatory, antitussive and/or anti-novel coronavirus;

• wherein the method comprising:
• (a) Preparing a traditional Chinese medicine composition, comprising the following steps:
• taking 18.9 parts by weight of Cornu caprae or 1.89 parts by weight of Cornu saigae tataricae, 9.45 parts u of Bulbus fritillariae ussuriensis, 6.3 parts by weight of Radix et Rhizoma rhei, 3.15 parts by weight of Radix scutellariae, 3.15 parts by weight of Lapis Chloriti, 4.724 parts by weight of Gypsum fibrosum, 1.89 parts by weight of Calculus bovis artifactus and 6.3 parts by weight of Radix glycyrrhizae;
• crushing the Cornu caprae or Cornu saigae tataricae into fine powder;
• hydrolyzing the powder with a sodium hydroxide-containing aqueous solution, filtering, and concentrating the filtrate;
• crushing the Lapis Chloriti and Gympsum fibrosum into crude powder;
• heating and decocting the crushed Lapis Chloriti and Gympsum fibrosum in water, filtering, and concentrating the filtrate;
• extracting the Calculus bovis artifactus with ethanol reflux, filtering, and concentrating the filtrate;
• decocting a rest of medicine in water, filtering, concentrating the filtrate and centrifuging, adding ethanol to the supernatant to precipitate;
• standing, taking the supernatant, filtering, recovering the ethanol under reduced pressure, concentrating, and combining the concentrated filtrate obtained above;
• (b) Detecting the active ingredients of the traditional Chinese medicine composition by conducting HPLC detection, wherein the chromatographic conditions comprise:
• COSMOSIL-C18 chromatographic column with specifications of 4.6 mm×250 mm, 5.0 um;
• mobile phase:
• methanol (B)-aqueous acid (A) (containing 0.1% formic acid), gradient elution:
• 0-15 min:
• 10-40% B, 15-70 min:
• 40-70% B, 70-90 min:
• 70-100% B;
• flow rate 0.8-1.2 mL/min, column temperature 30-40° C.;
• (c) Administrating the traditional Chinese medicine composition, when the detecting result of the active ingredients of the traditional Chinese medicine composition fall within the following:
• the active ingredients comprise by weight:
• 1610.45-1901.21 parts by weight of amino acids, 120.27-167.01 parts by weight of gallic acid, 136.22-172.23 parts by weight of liquiritin, 21.23-37.12 parts by weight of liquiritigenin, 411.30-542.50 parts by weight of baicalin, 40.54-55.01 parts by weight of oroxyloside, 121.11-148.22 parts by weight of wogonoside, 287.88-333.32 parts by weight of glycyrrhizic acid, 12.87-24.11 parts by weight of chrysin-7-O-β-D-glucoronic acid, 50.11-55.21 parts by weight of aloe-emodin 8-O-β-D-glucopyranoside, 30.71-38.56 parts by weight of chrysophanol-1-O-β-D-glucopyranoside, 45.09-57.88 parts by weight of chrysophanol-8-O-β-D-glucopyranoside, 260.11-285.32 parts by weight of hyodeoxycholic acid, 151.11-191.03 parts by weight of cholic acid.