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AssigneesPeking University Third Hospital, Institute of Chemistry, Chinese Academy of Sciences
InventorsJie Yan, Jie Qiao, Liying Yan, Rong Li, Jianjun Wang, Shenglin Jin, Jianyong Lv
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A cryopreservation solution contains 0.01-50.0 g of bionic ice control materials, 5.0-30 mL of polyols, 1-30 g of water-soluble sugar, and 0-30 mL of serum, and a buffer in every 100 mL of the cryopreservation solution. It does not contain DMSO. When being used for the cryopreservation of mouse oocytes and embryos, the solution may achieve the same or an even higher cell and tissue survival rate and functional expression stability as or than a commercial cryopreservation solution (containing 15% DMSO), and has high preservation efficiency. The cryopreservation solution without DMSO or serum reduces parasitic biological contaminants in the commercial cryopreservation solution containing serum currently used in clinical practice.
A DMSO-free cryopreservation solution, comprising, per 100 mL, 0.01-50 g of a biomimetic ice growth inhibition material, 5.0-45 ml of a polyalcohol, a water-soluble saccharide at 0.1-1.0 mol L−1, 0-30 mL of serum and the balance of a buffer, wherein the biomimetic ice growth inhibition material is selected from an atactic polyvinyl alcohol (PVA) having a syndiotacticity of 15%-60% and a molecular weight of from great than 10 kDa to 500 kDa, or a combination of said PVA with an amino acid biomimetic ice growth inhibition material.
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